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Research Article                                                                                             Online First
 
DOI:
The effect of allicin on the late sodium current associated with LQT3 syndromes caused by SCN5A mutation
Page view: 1211        
Authors:Xue–Bin CAO1;Chao ZHU2;Ying DONG2;Zhong–Qi CAI2;Hong–Lin WU2;Qiao XUE2;Yang LI2

Author Affiliation:1.Department of Cardiology, Chinese PLA General Hospital, Beijing, China; Department of Cardiology, the 252th hospitahospital of Chinese PLA, Baoding, China;2.Department of Cardiology, Chinese PLA General Hospital, Beijing, China;

Foundation:

Abstract: Objective We aimed to study the effect of the Allicin (All) on late sodium currents (INa,late) induced with several SCN5A genetic mutations, which were involved in long QT3 syndrome (LQT3).Methods WT and SCN5A mutation plasmids were transfected into human embryonic kidney (HEK293) cells. Patch-clamp technique was used in order to record Nav1.5 current and analyze the current affected by All. Results Compared with WT, significant increase of INa,late was observed from F1473S-SCN5A, T535I-SCN5A and E1784K-SCN5A. At -20 mV of test potential, the densities of INa,late were 3.12 ± 0.32 pA/pF of F1473S, 5.33 ± 0.56 pA/pF of T535I and 2.86 ± 0.23 pA/pF of E1784K, respectively. After exposure to 30 μM All, INa,late densities of SCN5A mutations were respectively reduced to 1.11 ± 0.05 pA/pF of F1473S, 0.9 ± 0.5 pA/pF of T535I and 1.0 ± 0.3 pA/pF of E1784K, close to the level of WT(0.3 ± 0.03 pA/pF). Furthermore, deactivation kinetic process of three SCN5A mutations was delayed with slow time constants lengthening of deactivation. Conclusions All decreases INa,late densities of SCN5A mutations with LQT3.

Keywords:

Allicin; Long QT3 syndrome; Late sodium currents; Patch clamp; SCN5A gene mutation
Received:December 13, 2017        Accepted:December 17, 2017   Published Online:December 22, 2017
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