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Laboratory Research
 
DOI:
Induction of macrophage inflammatory cytokines by Ox-LDL is ABCA1 dependent
Zhi-Gang Guo,Jian-Hua Li,Di Xie,Wen-Yan Lai,Jia-Yi Wu,Ping-Sheng Wu.J Geriatr Cardiol 2010,7(4):166~170
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Authors:Zhi-Gang Guo1;Jian-Hua Li1;Di Xie2;Wen-Yan Lai3;Jia-Yi Wu3;Ping-Sheng Wu3

Author Affiliation:1.Division of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China;2.Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China;3.Division of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China;

Foundation:

Abstract: Objective The current study aimed to evaluate whether the induction of macrophage inflammatory cytokines by Ox-LDL is related to the expression of ABCA1 pathway. Methods After THP1/PMA macrophages were transfected with ABCA1 antisense oligonucleotides (100nmol/L) followed by treatment with Ox-LDL (30mg/L), the expressions of ABCA1, ICAM-1 and MCP-1 mRNA and protein were determined by real-time fluorescent quantitative RT-PCR, Western blot or ELISA. Results Ox-LDL induced expressions of ABCA1, ICAM-1, and MCP-1 at both mRNA and protein levels from THP1/PMA macrophages. Transfection with ABCA1 antisense oligonucleotides reduced ABCA1 mRNA levels after 3 and 6 hours and protein levels after 12 and 24 hours. The expression of ICAM-1 and MCP-1 induced by Ox-LDL was also decreased after inhibition of ABCA1 protein expression by ABCA1 antisense oligonucleotide decreased. Conclusion The induction of macrophage inflammatory cytokines by Ox-LDL is partially dependent on expression of ABCA1. Our studies disclose new functions of ABCA1 in macrophages (J Geriatr Cardiol 2010; 7:166-170).

Keywords:

ATP-Binding cassette A1; THP1/PMA macrophage; inflammatory cytokine
          Published Online:December 28, 2010
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